
Proceedings Paper
DNA-templated nanoantennas for single-molecule detection at elevated concentrationsFormat | Member Price | Non-Member Price |
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Paper Abstract
The dynamic concentration range is one of the major limitations of single-molecule fluorescence
techniques. Here, we show how bottom-up nano-antennas enhance the fluorescence intensity in a
reduced hot-spot, ready for biological applications. We use self-assembled DNA origami structures as a
breadboard where gold nanoparticle dimers are positioned with nanometer precision. A maximum of
almost 100fold intensity enhancement is obtained using 100 nm gold nanoparticles within a gap of 23
nm between the particles. The results obtained are in good agreement with numerical simulations. Due
to the intensity enhancement introduced by the nano-antenna, we are able to perform single molecule
measurements at concentrations as high as 500 nM which represents an increment of 2 orders of
magnitude compared to conventional measurements. The combination of metallic nanoparticles with
DNA origami structures with docking points for biological assays paves the way for the development of
bottom-up inexpensive enhancement chambers for single molecule measurements at high
concentrations where processes like DNA sequencing occur.
Paper Details
Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 859509 (22 February 2013); doi: 10.1117/12.2003565
Published in SPIE Proceedings Vol. 8595:
Colloidal Nanocrystals for Biomedical Applications VIII
Wolfgang J. Parak; Marek Osinski; Kenji Yamamoto, Editor(s)
PDF: 8 pages
Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 859509 (22 February 2013); doi: 10.1117/12.2003565
Show Author Affiliations
G. P. Acuna, Technische Univ. Braunschweig (Germany)
P. Holzmeister, Technische Univ. Braunschweig (Germany)
F. M. Möller, Technische Univ. Braunschweig (Germany)
P. Holzmeister, Technische Univ. Braunschweig (Germany)
F. M. Möller, Technische Univ. Braunschweig (Germany)
S. Beater, Technische Univ. Braunschweig (Germany)
B. Lalkens, Technische Univ. Braunschweig (Germany)
P. Tinnefeld, Technische Univ. Braunschweig (Germany)
B. Lalkens, Technische Univ. Braunschweig (Germany)
P. Tinnefeld, Technische Univ. Braunschweig (Germany)
Published in SPIE Proceedings Vol. 8595:
Colloidal Nanocrystals for Biomedical Applications VIII
Wolfgang J. Parak; Marek Osinski; Kenji Yamamoto, Editor(s)
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