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Proceedings Paper

MUC1 aptamer based near infrared fluorescence probes for tumor diagnosis
Author(s): Juan Zhao; Yuxiang Ma; Sisi Cui; Jie Cao; Samuel Achilefu; Yueqing Gu
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Paper Abstract

Mucin 1 (MUC1) is a cell surface mucin broadly expressed in mucosal tissues. The aberrant expression of MUC1 under-glycosylated forms has been reported in various carcinomas of the epithelium, such as breast, pancreatic and ovarian cancers. Using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology, aptamers previously selected against MUC1 glycoprotein with high affinities and specificities. In this study, we developed two targeted near-infrared fluorescent probes for tumor in-vivo diagnostics using a MUC1 aptamer(APT) as targeted ligand and near-infrared fluorescent dye (ICG-Der-02) as labelling. MUC1 aptamer conjugated ICG-Der-02 (APT-ICG-Der-02) displayed a great selectivity to MUC1 positive cell line MCF7 and MCF7 xenograft-bearing nude mice. To improve the high targeting of the probe to the tumor cells, PEG, with high biocompatibility, non immunogenicity and long circulation, was conjugated to the probe .The new probe (APT-PEG-ICG-Der-02) showed better tumour uptake and clearance, and also displayed a great selectivity to MCF7 tumor-bearing nude mice. Data obtained demonstrate a high potential of the targeted near-infrared fluorescent probes in cancer early diagnosis.

Paper Details

Date Published: 21 February 2013
PDF: 9 pages
Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 859614 (21 February 2013); doi: 10.1117/12.2003364
Show Author Affiliations
Juan Zhao, China Pharmaceutical Univ. (China)
Yuxiang Ma, China Pharmaceutical Univ. (China)
Sisi Cui, China Pharmaceutical Univ. (China)
Jie Cao, China Pharmaceutical Univ. (China)
Samuel Achilefu, Washington Univ. School of Medicine in St. Louis (United States)
Yueqing Gu, China Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 8596:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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