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Proceedings Paper

High-spectral resolution nonlinear microspectroscopy and imaging of soft condensed and biological media
Author(s): Feruz Ganikhanov; Shan Yang; Sanjay Adhikari
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Paper Abstract

Precise information on dispersion of the nonlinear optical susceptibility of Raman active media is essential in order to get an insight into physics and chemistry of intra- and inter-molecular interactions. We propose and experimentally demonstrate a method that is capable of resolving both real and imaginary parts of third-order nonlinearity (χ(3)) in the vicinity of Raman resonances. Dispersion of χ(3) can be obtained from a medium probed within microscopic volumes with a spectral resolution of better than 0.1 cm-1 thus making our approach an essential tool in quantitative microscopic characterization of complex biological media. Time-domain CARS transients traced with femtosecond pulses within orders of magnitude in the signal decay can lead to resolution of fine spectral features in χ(3) dispersion that can not be reliably detected by frequency-domain Raman based spectroscopy/microscopy techniques, including coherent methods. We will present results of the method’s application in biological cells and tissue. Namely, we accessed a protein line at 1245 cm-1 in E-coli cell, major DNA and protein lines in red blood cells and triglyceride Raman active peaks in fat tissue.

Paper Details

Date Published: 19 March 2013
PDF: 6 pages
Proc. SPIE 8577, Optical Biopsy XI, 85770Q (19 March 2013); doi: 10.1117/12.2003240
Show Author Affiliations
Feruz Ganikhanov, West Virginia Univ. (United States)
Shan Yang, West Virginia Univ. (United States)
Sanjay Adhikari, West Virginia Univ. (United States)

Published in SPIE Proceedings Vol. 8577:
Optical Biopsy XI
Robert R. Alfano; Stavros G. Demos, Editor(s)

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