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Proceedings Paper

High-throughput optical injection of mammalian cells using a non-diffracting beam in a microfluidic platform
Author(s): Helen A. Rendall; Robert F. Marchington; Bavishna B. Praveen; Gerald Bergmann; Yoshihiko Arita; Alexander Heisterkamp; Frank J. Gunn-Moore; Kishan Dholakia
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Paper Abstract

Femtosecond photoporation is an optical, non-invasive method of injecting membrane impermeable substances contained within the surrounding medium into cells. The technique typically addresses individual cells in a static monolayer. While this gives excellent selectivity, it can be time consuming or impractical to treat larger samples. We build on previous work using a microfluidic platform, which allows for a suspension of cells to be dosed with femtosecond light as they flow through a microfluidic channel. A reusuable quartz chip is designed with an 's'-bend with facilitates the delivery of a 'non-diffracting' femtosecond Bessel beam along the centre of the channel. By implementing off-chip hydrodynamic focusing, cells are confined to the central region of the channel and pass along the Bessel beam core where they are photoporated. This new parallel approach allows for higher flow rates to be used compared to the previous, orthogonal, design whilst maintaining the necessary dwell time in the Bessel beam core. Optical injection of the cell membrane impermeable stain propidium iodide has been successful with two cell lines. These have yielded viable injection efficiencies of 31.0±9.5% Chinese hamster ovary cells (CHO-K1) and 20.4±4.2% human promyelocytic cells (HL60) with a cell throughput of up to 10 cells/second. This marks an order of magnitude increase compared to the previous microfluidic design.

Paper Details

Date Published: 15 March 2013
PDF: 7 pages
Proc. SPIE 8611, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII, 861103 (15 March 2013); doi: 10.1117/12.2003193
Show Author Affiliations
Helen A. Rendall, Univ. of St. Andrews (United Kingdom)
Robert F. Marchington, Univ. of St. Andrews (United Kingdom)
Bavishna B. Praveen, Univ. of St. Andrews (United Kingdom)
Gerald Bergmann, Friedrich-Schiller-Univ. Jena (Germany)
Yoshihiko Arita, Univ. of St. Andrews (United Kingdom)
Alexander Heisterkamp, Friedrich-Schiller-Univ. Jena (Germany)
Frank J. Gunn-Moore, Univ. of St. Andrews (United Kingdom)
Kishan Dholakia, Univ. of St. Andrews (United Kingdom)

Published in SPIE Proceedings Vol. 8611:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte, Editor(s)

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