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Proceedings Paper

Optical clearing and multiphoton imaging of paraffin-embedded specimens
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Paper Abstract

New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffin-embedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858813 (22 February 2013); doi: 10.1117/12.2003155
Show Author Affiliations
Jesse W. Wilson, Duke Univ. (United States)
Simone Degan, Duke Univ. (United States)
Martin C. Fischer, Duke Univ. (United States)
Warren S. Warren, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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