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Proceedings Paper

Imaging pigment chemistry in melanocytic conjunctival lesions with pump-probe microscopy
Author(s): Jesse W. Wilson; Lejla Vajzovic; Francisco E. Robles; Thomas J. Cummings; Prithvi Mruthyunjaya; Warren S. Warren
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Paper Abstract

We extend nonlinear pump-probe microscopy, recently demonstrated to image the microscopic distribution of eumelanin and pheomelanin in unstained skin biopsy sections, to the case of melanocytic conjunctival lesions. The microscopic distribution of pigmentation chemistry serves as a functional indicator of melanocyte activity. In these conjunctival specimens (benign nevi, primary acquired melanoses, and conjunctival melanoma), we have observed pump-probe spectroscopic signatures of eumelanin, pheomelanin, hemoglobin, and surgical ink, in addition to important structural features that differentiate benign from malignant lesions. We will also discuss prospects for an in vivo ‘optical biopsy’ to provide additional information before having to perform invasive procedures.

Paper Details

Date Published: 26 March 2013
PDF: 7 pages
Proc. SPIE 8567, Ophthalmic Technologies XXIII, 856718 (26 March 2013); doi: 10.1117/12.2003137
Show Author Affiliations
Jesse W. Wilson, Duke Univ. (United States)
Lejla Vajzovic, Duke Univ. (United States)
Francisco E. Robles, Duke Univ. (United States)
Thomas J. Cummings, Duke Univ. (United States)
Prithvi Mruthyunjaya, Duke Univ. (United States)
Warren S. Warren, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 8567:
Ophthalmic Technologies XXIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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