
Proceedings Paper
Two-photon-based structured illumination microscopy applied for superresolution optical biopsyFormat | Member Price | Non-Member Price |
---|---|---|
$17.00 | $21.00 |
Paper Abstract
Combining the concept of structured illumination with two-photon microscopy, two-photon grid scanning pattern
microscopy (TP-GPSM) was demonstrated to provide optical section power due to two-photon excitation and super-resolution
in lateral through structured illumination. Based on a laser scanning geometry, the two-photon illumination
patterns were effectively produced by temporally and spatially modulating the excitation light. Several possible ways to
produce structured patterns were proposed in this paper. For image reconstruction, sufficient phase-stepped images were
needed. With the 2-dimensional grid scanning pattern, ten images, including two orientations and five images with
different phases in each orientation are required, and a 2-fold improvement in lateral resolution can be obtained. TP-GPSM
was shown to have the potential for super-resolution imaging in thick tissues.
Paper Details
Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858826 (22 February 2013); doi: 10.1117/12.2003028
Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)
PDF: 8 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858826 (22 February 2013); doi: 10.1117/12.2003028
Show Author Affiliations
Chia Hua Yeh, National Central Univ. (Taiwan)
Szu Yu Chen, National Central Univ. (Taiwan)
Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)
© SPIE. Terms of Use
