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Proceedings Paper

Single molecule interactions studied by using a modified DNA sequencer: a comparison with surface plasmon resonance
Author(s): Jens Sobek; Stefan Schauer; Hubert Rehrauer; David Fischer; Andrea Patrignani; Stephan Landgraf; Ralph Schlapbach
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Paper Abstract

In this study we established the principles for using a commercial off-the-shelf DNA sequencer (RS, Pacific Biosciences) to measure biomolecular interactions. Binding between single oligonucleotides immobilized at the bottom of a nanowell to a complementary DNA strand was used as a model system. The influence of the labeling dye on the hybridization of a 7mer oligonucleotide was investigated. The resulting association and dissociation rate constants (kon and koff) and the calculated dissociation constants (Kd) were compared to data obtained from surface plasmon resonance (SPR) measurements. In the present study we identified a good agreement of the determined kinetic constants by the two methods investigated.

Paper Details

Date Published: 21 February 2013
PDF: 10 pages
Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960Y (21 February 2013); doi: 10.1117/12.2002953
Show Author Affiliations
Jens Sobek, ETH Zurich (Switzerland)
Stefan Schauer, ETH Zurich (Switzerland)
Hubert Rehrauer, ETH Zurich (Switzerland)
David Fischer, ETH Zurich (Switzerland)
Andrea Patrignani, ETH Zurich (Switzerland)
Stephan Landgraf, Graz Univ. of Technology (Austria)
Ralph Schlapbach, ETH Zurich (Switzerland)

Published in SPIE Proceedings Vol. 8596:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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