
Proceedings Paper
Atomic force imaging microscopy investigation of the interaction of ultraviolet radiation with collagen thin filmsFormat | Member Price | Non-Member Price |
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Paper Abstract
Collagen is the major fibrous protein in the extracellular matrix and consists a significant component of skin, bone,
cartilage and tendon. Due to its unique properties, it has been widely used as scaffold or culture substrate for tissue
regeneration or/and cell-substrate interaction studies. The ultraviolet light-collagen interaction investigations are crucial
for the improvement of many applications such as that of the UV irradiation in the field of biomaterials, as sterilizing and
photo-cross-linking method. The aim of this paper was to investigate the mechanisms of UV-collagen interactions by
developing a collagen-based, well characterized, surface with controlled topography of collagen thin films in the
nanoscale range. The methodology was to quantify the collagen surface modification induced on ultraviolet radiation and
correlate it with changes induced in cells. Surface nanoscale characterization was performed by Atomic Force
Microscopy (AFM) which is a powerful tool and offers quantitative and qualitative information with a non-destructive
manner. In order to investigate cells behavior, the irradiated films were used for in vitro cultivation of human skin
fibroblasts and the cells morphology, migration and alignment were assessed with fluorescence microscopy imaging and
image processing methods. The clarification of the effects of UV light on collagen thin films and the way of cells
behavior to the different modifications that UV induced to the collagen-based surfaces will contribute to the better
understanding of cell-matrix interactions in the nanoscale and will assist the appropriate use of UV light for developing
biomaterials.
Paper Details
Date Published: 19 February 2013
PDF: 15 pages
Proc. SPIE 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940E (19 February 2013); doi: 10.1117/12.2002460
Published in SPIE Proceedings Vol. 8594:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)
PDF: 15 pages
Proc. SPIE 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940E (19 February 2013); doi: 10.1117/12.2002460
Show Author Affiliations
A. Stylianou, National Technical Univ. of Athnes (Greece)
D. Yova, National Technical Univ. of Athnes (Greece)
D. Yova, National Technical Univ. of Athnes (Greece)
E. Alexandratou, National Technical Univ. of Athnes (Greece)
A. Petri, National Technical Univ. of Athnes (Greece)
A. Petri, National Technical Univ. of Athnes (Greece)
Published in SPIE Proceedings Vol. 8594:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)
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