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Proceedings Paper

Computational analysis of the spatial distribution of mitotic spindle angles in mouse developing airway
Author(s): Nan Tang; Wallace F. Marshall
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Paper Abstract

Investigating the spatial information of cellular processes in tissues during mouse embryo development is one of the major technical challenges in development biology. Many imaging methods are still limited to the volumes of tissue due to tissue opacity, light scattering and the availability of advanced imaging tools. For analyzing the mitotic spindle angle distribution in developing mouse airway epithelium, we determined spindle angles in mitotic epithelial cells on serial sections of whole airway of mouse embryonic lungs. We then developed a computational image analysis to obtain spindle angle distribution in three dimensional airway reconstructed from the data obtained from all serial sections. From this study, we were able to understand how mitotic spindle angles are distributed in a whole airway tube. This analysis provides a potentially fast, simple and inexpensive alternative method to quantitatively analyze cellular process at subcellular resolution. Furthermore, this analysis is not limited to the size of tissues, which allows to obtain three dimensional and high resolution information of cellular processes in cell populations deeper inside intact organs.

Paper Details

Date Published: 26 March 2013
PDF: 6 pages
Proc. SPIE 8593, Optical Methods in Developmental Biology, 85930N (26 March 2013); doi: 10.1117/12.2002240
Show Author Affiliations
Nan Tang, National Institute of Biological Science (China)
Univ. of California, San Francisco (United States)
Wallace F. Marshall, Univ. of California, San Francisco (United States)

Published in SPIE Proceedings Vol. 8593:
Optical Methods in Developmental Biology
Andrew M. Rollins; Cecilia Lo; Scott E. Fraser, Editor(s)

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