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Proceedings Paper

Aptamer-based surface plasmon resonance sensing of glycated human blood proteins
Author(s): Nathan G. F. Reaver; Rui Zheng; Dong-Shik Kim; Brent D. Cameron
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Paper Abstract

The concentration ratio of glycated to non-glycated forms of various blood proteins can be used as a diagnostic measure in diabetes to determine a history of glycemic compliance. Depending on a protein’s half-life in blood, compliance can be assessed from a few days to several months in the past, which can then be used to provide additional therapeutic guidance. Current glycated protein detection methods are limited in their ability to measure multiple proteins, and are susceptible to interference from other blood pathologies. In this study, we developed and characterized DNA aptamers for use in Surface Plasmon Resonance (SPR) sensors to assess the blood protein hemoglobin. The aptamers were developed by way of a modified Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process which selects DNA sequences that have a high binding affinity to a specific protein. DNA products resulting from this process are sequenced and identified aptamers are then synthesized. The SELEX process was performed to produce aptamers for a glycated form of hemoglobin. Equilibrium dissociation constants for the binding of the identified aptamer to glycated hemoglobin, hemoglobin, and fibrinogen were calculated from fitted Langmuir isotherms obtained through SPR. These constants were determined to be 94 nM, 147 nM, and 244 nM respectively. This aptamer can potentially be used to create a SPR aptamer based biosensor for detection of glycated hemoglobin, a technology that has the potential to deliver low-cost and immediate glycemic compliance assessment in either a clinical or home setting.

Paper Details

Date Published: 21 February 2013
PDF: 12 pages
Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 85970G (21 February 2013); doi: 10.1117/12.2002086
Show Author Affiliations
Nathan G. F. Reaver, The Univ. of Toledo (United States)
Rui Zheng, The Univ. of Toledo (United States)
Dong-Shik Kim, The Univ. of Toledo (United States)
Brent D. Cameron, The Univ. of Toledo (United States)

Published in SPIE Proceedings Vol. 8597:
Plasmonics in Biology and Medicine X
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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