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Proceedings Paper

Development of optics with micro-LED arrays for improved opto-electronic neural stimulation
Author(s): Lionel Chaudet; Mark Neil; Patrick Degenaar; Kamyar Mehran; Rolando Berlinguer-Palmini ; Brian Corbet; Pleun Maaskant; David Rogerson; Peter Lanigan; Ernst Bamberg; Botond Roska
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Paper Abstract

The breakthrough discovery of a nanoscale optically gated ion channel protein, Channelrhodopsin 2 (ChR2), and its combination with a genetically expressed ion pump, Halorhodopsin, allowed the direct stimulation and inhibition of individual action potentials with light alone. This work reports developments of ultra-bright elec­ tronically controlled optical array sources with enhanced light gated ion channels and pumps for use in systems to further our understanding of both brain and visual function. This work is undertaken as part of the European project, OptoNeuro. Micro-LED arrays permit spatio-temporal control of neuron stimulation on sub-millisecond timescales. However they are disadvantaged by their broad spatial light emission distribution and low fill factor. We present the design and implementation of a projection and micro-optics system for use with a micro-LED array consisting of a 16x16 matrix of 25 μm diameter micro-LEDs with 150 μm centre-to-centre spacing and an emission spectrum centred at 470 nm overlapping the peak sensitivity of ChR2 and its testing on biological samples. The projection system images the micro-LED array onto micro-optics to improve the fill-factor from ~2% to more than 78% by capturing a larger fraction of the LED emission and directing it correctly to the sample plane. This approach allows low fill factor arrays to be used effectively, which in turn has benefits in terms of thermal management and electrical drive from CMOS backplane electronics. The entire projection system is integrated into a microscope prototype to provide stimulation spots at the same size as the neuron cell body (μ10 pm).

Paper Details

Date Published: 8 March 2013
PDF: 7 pages
Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860R (8 March 2013); doi: 10.1117/12.2002070
Show Author Affiliations
Lionel Chaudet, Imperial College London (United Kingdom)
Mark Neil, Imperial College London (United Kingdom)
Patrick Degenaar, Newcastle Univ. (United Kingdom)
Kamyar Mehran, Newcastle Univ. (United Kingdom)
Rolando Berlinguer-Palmini , Newcastle Univ. (United Kingdom)
Brian Corbet, National Tyndall Institute (Ireland)
Pleun Maaskant, National Tyndall Institute (Ireland)
David Rogerson, Scientifica (United Kingdom)
Peter Lanigan, Scientifica (United Kingdom)
Ernst Bamberg, Max Planck Institute of Biophysics (Germany)
Botond Roska, Friederich Miescher Institut (Switzerland)

Published in SPIE Proceedings Vol. 8586:
Optogenetics: Optical Methods for Cellular Control
Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)

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