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Proceedings Paper

Multiphoton cryo microscope with sample temperature control
Author(s): H. G. Breunig; A. Uchugonova; K. König
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Paper Abstract

We present a multiphoton microscope system which combines the advantages of multiphoton imaging with precise control of the sample temperature. The microscope provides online insight in temperature-induced changes and effects in plant tissue and animal cells with subcellular resolution during cooling and thawing processes. Image contrast is based on multiphoton fluorescence intensity or fluorescence lifetime in the range from liquid nitrogen temperature up to +600°C. In addition, micro spectra from the imaged regions can be recorded. We present measurement results from plant leaf samples as well as Chinese hamster ovary cells.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858819 (22 February 2013); doi: 10.1117/12.2001994
Show Author Affiliations
H. G. Breunig, JenLab GmbH (Germany)
Saarland Univ. (Germany)
A. Uchugonova, JenLab GmbH (Germany)
K. König, JenLab GmbH (Germany)
Saarland Univ. (Germany)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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