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Proceedings Paper

Whispering gallery mode aptasensors for detection of blood proteins
Author(s): L. Pasquardini; S. Berneschi; A. Barucci; F. Cosi; M. Insinna; L. Lunelli; G. Nunzi Conti; C. Pederzolli; S. Salvadori; S. Soria
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Paper Abstract

Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was numerically modelled and optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.

Paper Details

Date Published: 20 March 2013
PDF: 8 pages
Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 85760U (20 March 2013); doi: 10.1117/12.2001987
Show Author Affiliations
L. Pasquardini, Fondazione Bruno Kessler Ctr. for Materials and Microsystems (Italy)
S. Berneschi, Ctr. Studi e Ricerche E. Fermi (Italy)
Nello Carrara Institute of Applied Physics, CNR (Italy)
A. Barucci, Nello Carrara Institute of Applied Physics, CNR (Italy)
F. Cosi, Nello Carrara Institute of Applied Physics, CNR (Italy)
M. Insinna, Univ. degli Studi di Firenze (Italy)
L. Lunelli, Fondazione Bruno Kessler Ctr. for Materials and Microsystems (Italy)
IBF-CNR, Univ. degli Studi di Trento (Italy)
G. Nunzi Conti, Nello Carrara Institute of Applied Physics, CNR (Italy)
C. Pederzolli, Fondazione Bruno Kessler Ctr. for Materials and Microsystems (Italy)
S. Salvadori, Univ. degli Studi di Firenze (Italy)
S. Soria, Nello Carrara Institute of Applied Physics, CNR (Italy)


Published in SPIE Proceedings Vol. 8576:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII
Israel Gannot, Editor(s)

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