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Proceedings Paper

Combining multiphoton and CARS microscopy for skin imaging
Author(s): H. G. Breunig; M. Weinigel; M. Kellner-Höfer; R. Bückle; M. E. Darvin; J. Lademann; K. König
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Paper Abstract

Microscopic imaging based on multiphoton fluorescence, second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging has been realized in one common platform which is appropriate for use in hospitals. The different optical modalities non-invasively provide in vivo images from human skin with subcellular resolution, at different depths based on endogenous fluorescent, SHG-active molecules as well as non-fluorescent molecules with vibrational resonances at 2845 cm-1, in particular lipids. An overview of the system employing a Ti:sapphire laser and photonic crystal fiber to generate the excitation light as well as several imaging examples are presented.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880N (22 February 2013); doi: 10.1117/12.2001985
Show Author Affiliations
H. G. Breunig, JenLab GmbH (Germany)
Saarland Univ. (Germany)
M. Weinigel, JenLab GmbH (Germany)
M. Kellner-Höfer, JenLab GmbH (Germany)
R. Bückle, JenLab GmbH (Germany)
M. E. Darvin, Charité Universitätsmedizin Berlin (Germany)
J. Lademann, Charité Universitätsmedizin Berlin (Germany)
K. König, JenLab GmbH (Germany)
Saarland Univ. (Germany)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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