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Proceedings Paper

High-speed multispectral confocal imaging
Author(s): Gary E. Carver; Sarah A. Locknar; William A. Morrison; Daniel L. Farkas
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Paper Abstract

A new approach for generating high-speed multispectral images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This concept merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described, and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.

Paper Details

Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858715 (22 February 2013); doi: 10.1117/12.2001883
Show Author Affiliations
Gary E. Carver, Omega Optical Inc. (United States)
Sarah A. Locknar, Omega Optical Inc. (United States)
William A. Morrison, Omega Optical Inc. (United States)
Daniel L. Farkas, Spectral Molecular Imaging (United States)

Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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