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Proceedings Paper

Optical metabolic imaging of live tissue cultures
Author(s): Alex J. Walsh; Rebecca S. Cook; Carlos L. Arteaga; Melissa C. Skala
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Paper Abstract

The fluorescence properties, both intensity and fluorescence lifetime, of NADH and FAD, two coenzymes of metabolism, are sensitive, high resolution measures of cellular metabolism. However, often in vivo measurements of tissue are not feasible. In this study, we investigate the stability over time of two-photon auto-fluorescence imaging of NADH and FAD in live-cultured tissues. Our results demonstrate that cultured tissues remain viable for at least several days post excision. Furthermore, the optical redox ratio, NADH fluorescence lifetime, and FAD fluorescence lifetime do not significantly change in the cultured tissues over time. With these findings, we demonstrate the potential of sustained tissue culture techniques for optical metabolic imaging.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858820 (22 February 2013); doi: 10.1117/12.2001863
Show Author Affiliations
Alex J. Walsh, Vanderbilt Univ. (United States)
Rebecca S. Cook, Vanderbilt Univ. (United States)
Carlos L. Arteaga, Vanderbilt Univ. (United States)
Vanderbilt Univ. Ingram Cancer Ctr. (United States)
Melissa C. Skala, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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