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Proceedings Paper

Clinical multiphoton endoscopy with FLIM capability
Author(s): Martin Weinigel; Hans Georg Breunig; Peter Fischer; Marcel Kellner-Höfer; Rainer Bückle; Karsten König
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Paper Abstract

Multiphoton endoscopy can be applied for intra-corporeal imaging as well as to examine otherwise hard-to-access tissue areas like chronic wounds. Using high-NA (NA = 0.8) gradient-index (GRIN) lens-based endoscopes with a diameter of 1.4 mm and effective lengths of 7 mm and 20 mm, respectively, two-photon excitation of endogenous fluorophores and second-harmonic generation (SHG) is used for multimodal in vivo imaging of human skin. A further imaging modality is fluorescence lifetime imaging (FLIM) which allows functional imaging to investigate the healing mechanism of chronic wounds and the corresponding cell metabolism. We performed first in vivo measurements using FLIM endoscopy with the medically-certified multiphoton tomograph MPTflex® in combination with a computer-controlled motorized scan head and a GRIN-lens endoscope.

Paper Details

Date Published: 22 February 2013
PDF: 9 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882E (22 February 2013); doi: 10.1117/12.2001849
Show Author Affiliations
Martin Weinigel, JenLab GmbH (Germany)
Hans Georg Breunig, JenLab GmbH (Germany)
Saarland Univ. (Germany)
Peter Fischer, JenLab GmbH (Germany)
Marcel Kellner-Höfer, JenLab GmbH (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Karsten König, JenLab GmbH (Germany)
Saarland Univ. (Germany)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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