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Proceedings Paper

Role of the amyloid region in the formation and propagation of Als adhesive nanodomains on Candida albicans
Author(s): David Alsteens
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Paper Abstract

The most prevalent fungal pathogen in humans Candida albicans expresses on its surface Als (Agglutinin-like sequence) proteins which mediate both yeast-to-host tissue adherence and yeast aggregation. Although adhesins often show weak binding to specific ligands, Als mediate remarkably strong adherence. By combining single-molecule atomic force microscopy (AFM) with the tools of genetics and cell biology, we unraveled the various Als protein domains to understand how they synergize to strengthen the cell adhesion and aggregation. The complementary role of the ligandbinding domains, amyloid-forming regions and the less specific hydrophobic tandem repeat domains give raise to a fascinating new mechanism of cell-activation resulting in force-activated clustering of hundreds of adhesion molecules. The amyloid heptapeptide sequence plays a crucial role in this mechanism providing a cohesive strength to the Als protein by forming molecular zipper that mediates protein interactions between cells.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900F (22 February 2013); doi: 10.1117/12.2001773
Show Author Affiliations
David Alsteens, Univ. Catholique de Louvain (Belgium)

Published in SPIE Proceedings Vol. 8590:
Single Molecule Spectroscopy and Superresolution Imaging VI
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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