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Proceedings Paper

Imaging plant tissues with coherent Raman scattering microscopy
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Paper Abstract

Microscopic chemical mapping of living plant tissues without the use of extrinsic labels would represent a major advance in analytical capability for many areas of biological research; Coherent Raman Scattering (CRS) microscopy offers label-free chemical imaging based on vibrational spectroscopy and is an obvious solution. However, due to the high levels of optical absorption and fluorescent emission in plant tissues the technique is severely limited for in-vivo plant imaging. This paper reports preliminary results regarding the technical issues associated with performing label-free imaging in plant tissues with CRS and discusses how they may be mitigated in future applications.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880I (22 February 2013); doi: 10.1117/12.2001696
Show Author Affiliations
J. Moger, Univ. of Exeter (United Kingdom)
J. C. Mansfield, Univ. of Exeter (United Kingdom)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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