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Proceedings Paper

O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles
Author(s): Serena Mazzucchelli; Miriam Colombo; Elisabetta Galbiati; Fabio Corsi; Josè M. Montenegro; Wolfgang J. Parak; Davide Prosperi
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Paper Abstract

A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the biologically mediated site-specific covalent conjugation of targeting proteins to nanoparticles. ScFv800E6, an scFv mutant selective for HER2 antigen overexpressed in breast cancer cells was chosen as targeting ligand. The fusion protein SNAP-scFv was irreversibly immobilized on magnetofluorescent nanoparticles through the recognition between SNAP module and pegylated O6-alkylguanine derivative. The targeting efficiency of the resulting nanoparticle against HER2-positive breast cancer cells was assessed by flow cytometry and immunofluorescence.

Paper Details

Date Published: 22 February 2013
PDF: 9 pages
Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 859502 (22 February 2013); doi: 10.1117/12.2001648
Show Author Affiliations
Serena Mazzucchelli, Sacco Hospital (Italy)
Miriam Colombo, Univ. of Milano-Bicocca (Italy)
Elisabetta Galbiati, Univ. of Milano-Bicocca (Italy)
Fabio Corsi, Sacco Hospital (Italy)
Josè M. Montenegro, Philipps-Univ. Marburg (Germany)
Wolfgang J. Parak, Philipps-Univ. Marburg (Germany)
Davide Prosperi, Univ. of Milano-Bicocca (Italy)

Published in SPIE Proceedings Vol. 8595:
Colloidal Nanocrystals for Biomedical Applications VIII
Wolfgang J. Parak; Marek Osinski; Kenji Yamamoto, Editor(s)

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