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Proceedings Paper

Nanoscale surface cues and in vitro neuronal growth
Author(s): Yoonkey Nam; Min Jee Jang; Kyungtae Kang; Insung S. Choi
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Paper Abstract

Nerve cells (neurons) have been used for a convenient and effective model for basic neurobiology and it has also served as a test bed for the development of neural prosthetic devices. The characterization of neuronal growth in vitro has become an important part of neural tissue engineering. In this talk, I will present recent progresses on the investigation of nano-scale effects on neuronal growth in vitro. Hippocampal neurons from a small brain tissue dissected from E-18 (embryonic stage 18 days) Sprague-Dawley rat were used as a developing neuron model. They were seeded on substrates with carbon nanotube patterned glass substrates, anodized aluminum oxide surfaces with two different pitch sizes (60 nm, 400 nm), and silica nano bead surfaces with five different bead sizes (110, 190, 320, 480, 670 nm). These surfaces uniquely defined nano-scale surfaces with different topographical features. We observed longer neurite outgrowth and faster neuronal development on nano-scale surfaces compared to plain glass surfaces. The results from nano-scale cell culture platforms will be useful to understand nano-environments of the brain during the early neural developments. In addition, the promoted neuronal development could be further applied for neural tissue scaffolds or implantable neural interface systems.

Paper Details

Date Published: 24 October 2012
PDF: 6 pages
Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 854843 (24 October 2012); doi: 10.1117/12.2001590
Show Author Affiliations
Yoonkey Nam, KAIST (Korea, Republic of)
Min Jee Jang, KAIST (Korea, Republic of)
Kyungtae Kang, KAIST (Korea, Republic of)
Insung S. Choi, KAIST (Korea, Republic of)

Published in SPIE Proceedings Vol. 8548:
Nanosystems in Engineering and Medicine
Sang H. Choi; Jin-Ho Choy; Uhn Lee M.D.; Vijay K. Varadan, Editor(s)

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