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Proceedings Paper

How low-level laser therapy can change mechanical properties of cells
Author(s): Ana Carolina de Magalhães; Diana Martinez; Marcia Z. J. Ferreira; Ellisabeth M. Yoshimura; Adriano M. Alencar; Maria Cristina Chavantes
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Paper Abstract

Low level laser therapy is used as a treatment of several conditions, including inflammatory processes and wound healing. Possible changes in mechanical properties of cells, caused by illumination, are investigated with optical magnetic twisting cytometry (OMTC), which is a technique used to evaluate mechanical properties in cell culture. Ferromagnetic micro beads are bound to cell cytoskeleton, the beads are magnetized vertically and a horizontal twisting magnetic field is applied causing a torque that moves the beads and deforms the cell, the beads rotate and displace. Based on the lateral displacement of the beads, elastic shear and loss moduli are obtained. Samples of human bronchial epithelial cell culture were divided in two groups: one was illuminated with a 660 nm red laser, 30 mW power, 0.75 W/cm2 irradiance, during different time intervals, and the other one, the control group, was not illuminated. The values of the mechanical constants of the cells of the control group showed a tendency of increasing with the time out of the incubator. On the other hand, the illuminated group showed constancy on the behavior of both moduli, keeping the normal conditions of the cell culture. Those results indicate that illumination can induce cells to homeostasis, and OMTC is sensitive to observe departures from the steady conditions. Hence, OMTC is an important technique which can be used to aggregate knowledge on the light effect in cell cytoskeleton and even on the low level laser therapy mechanisms in inflammatory processes and/or wound healing.

Paper Details

Date Published: 5 March 2013
PDF: 6 pages
Proc. SPIE 8569, Mechanisms for Low-Light Therapy VIII, 856909 (5 March 2013); doi: 10.1117/12.2001553
Show Author Affiliations
Ana Carolina de Magalhães, Univ. de São Paulo (Brazil)
Diana Martinez, Univ. de São Paulo Medical School (Brazil)
Marcia Z. J. Ferreira, Univ. Estadual de Campinas (Brazil)
Ellisabeth M. Yoshimura, Univ. de São Paulo (Brazil)
Adriano M. Alencar, Univ. de São Paulo Medical School (Brazil)
Maria Cristina Chavantes, Univ. de São Paulo Medical School (Brazil)

Published in SPIE Proceedings Vol. 8569:
Mechanisms for Low-Light Therapy VIII
Michael R. Hamblin; Juanita Anders; James D. Carroll; Praveen R. Arany, Editor(s)

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