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Proceedings Paper

The arrangement of fibrous collagen in cornea using second harmonic generation (SHG) microscopy
Author(s): Yair Mega; James McLean; Ramin Zareian; Stephen Karasek; Zhenhua Lai; Charles DiMarzio
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Paper Abstract

In recent years, interest in studying the components of the cornea and their arrangement, with emphasis on the corneal stroma, has expanded rapidly. By determining the corneal stroma’s organization in detail, we will understand better the relationship between its structure and functionality. Here, the cornea’s collagen lamellae were scanned using second harmonic generation (SHG) microscopy, in order to determine the orientation of fibers in different directions within a two-dimensional cross section. A unique algorithm was used to quantitatively measure the directions. Cross sections were obtained at several different depths in each sample. This work offers supplemental sectioning revelations to the methods historically used to scan at the lamella level, such as X-ray diffraction.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881N (22 February 2013); doi: 10.1117/12.2001551
Show Author Affiliations
Yair Mega, Northeastern Univ. (United States)
James McLean, Northeastern Univ. (United States)
Ramin Zareian, Northeastern Univ. (United States)
Stephen Karasek, Northeastern Univ. (United States)
Zhenhua Lai, Northeastern Univ. (United States)
Charles DiMarzio, Northeastern Univ. (United States)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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