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Proceedings Paper

Polymeric rapid prototyping for inexpensive and portable medical diagnostics
Author(s): Tianchi Ma; Victoria Northrup; Andrew O. Fung; D. Moira Glerum; Christopher J. Backhouse
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Paper Abstract

The advent of inexpensive CO2 laser systems has led to a wide range of demonstrations of microfabricated lab on chip systems built of acrylic. However, there has been little application of these systems to building microfluidics for DNA analysis. In this work we explore the use of CO2 laser systems for building microfluidics for DNA analysis and relate the artifacts of the fabrication technology to the performance of the system. We show that surface roughness that leads to significant constrictions in the separation channel provides an upper limit of the size of DNA that can be analysed. Below that upper limit, the resolution of the chip is strongly affected by the degree to which the separation channel is exposed to redeposited by-products of the ablation process. We show that by controlling these effects we are reliably able to discern two types of PCR product as a test representative of a real application. By being able to do this is in microfluidic devices the size of a postage stamp we have shown that we can now use CO2 laser systems for the development of extremely inexpensive diagnostic systems using a rapid prototyping approach.

Paper Details

Date Published: 24 October 2012
PDF: 8 pages
Proc. SPIE 8412, Photonics North 2012, 84120B (24 October 2012);
Show Author Affiliations
Tianchi Ma, Univ. of Waterloo (Canada)
Victoria Northrup, Univ. of Alberta (Canada)
Andrew O. Fung, Univ. of Waterloo (Canada)
D. Moira Glerum, Univ. of Waterloo (Canada)
Univ. of Alberta (Canada)
Christopher J. Backhouse, Univ. of Waterloo (Canada)

Published in SPIE Proceedings Vol. 8412:
Photonics North 2012
Jean-Claude Kieffer, Editor(s)

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