In this work, we utilize digital holographic microscopy technique and also conventional video microscopy to detect the dynamic morphological changes of bacteria membrane in presence of silver ions. Silver ions have shown strong inhibitory effects on bacteria and can be used as antibacterial. We used E. coli as a sample and the influences of the ions were compared in terms of the variations in volume of E. coli for different concentration of silver ions in the buffer and various incubation times. In a controlled experiment using a microinjecting pump the concentration of antibacterial is increased, the movement of a single or a set of bacteria are monitored live, and successive digital holograms are recorded. The recorded holograms by digital camera can be post-processed to three dimensional reconstruction of the samples and measurement of quantitative structural changes in various interacting time of the bacteria.

Date Published: 26 November 2012
PDF: 6 pages
Proc. SPIE 8556, Holography, Diffractive Optics, and Applications V, 85560N (26 November 2012); doi: 10.1117/12.2001370

Published in SPIE Proceedings Vol. 8556:
Holography, Diffractive Optics, and Applications V
Yunlong Sheng; Chongxiu Yu; Linsen Chen, Editor(s)