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Proceedings Paper

Label-free imaging of the dynamics of cell-to-cell string-like structure bridging in the free-space by low-coherent quantitative phase microscopy
Author(s): Toyohiko Yamauchi; Hidenao Iwai; Yutaka Yamashita
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Paper Abstract

We succeeded in utilizing our low-coherent quantitative phase microscopy (LC-QPM) to achieve label-free and three-dimensional imaging of string-like structures bridging the free-space between live cells. In past studies, three dimensional morphology of the string-like structures between cells had been investigated by electron microscopies and fluorescence microscopies and these structures were called ”membrane nanotubes” or “tunneling nanotubes.” However, use of electron microscopy inevitably kills these cells and fluorescence microscopy is itself a potentially invasive method. To achieve noninvasive imaging of live cells, we applied our LC-QPM which is a reflection-type, phase resolved and full-field interference microscope employing a low-coherent light source. LC-QPM is able to visualize the three-dimensional morphology of live cells without labeling by means of low-coherence interferometry. The lateral (diffraction limit) and longitudinal (coherence-length) spatial resolution of LC-QPM were respectively 0.49 and 0.93 micrometers and the repeatability of the phase measurement was 0.02 radians (1.0 nm). We successfully obtained three-dimensional morphology of live cultured epithelial cells (cell type: HeLa, derived from cervix cancer) and were able to clearly observe the individual string-like structures interconnecting the cells. When we performed volumetric imaging, a 80 micrometer by 60 micrometer by 6.5 micrometer volume was scanned every 5.67 seconds and 70 frames of a three-dimensional movie were recorded for a duration of 397 seconds. Moreover, the optical phase images gave us detailed information about the three-dimensional morphology of the string-like structure at sub-wavelength resolution. We believe that our LC-QPM will be a useful tool for the study of three-dimensional morphology of live cells.

Paper Details

Date Published: 20 March 2013
PDF: 8 pages
Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 85711G (20 March 2013); doi: 10.1117/12.2000674
Show Author Affiliations
Toyohiko Yamauchi, Hamamatsu Photonics K.K. (Japan)
Hidenao Iwai, Hamamatsu Photonics K.K. (Japan)
Yutaka Yamashita, Hamamatsu Photonics K.K. (Japan)

Published in SPIE Proceedings Vol. 8571:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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