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Proceedings Paper

Near-infrared spectroscopy of renal tissue in vivo
Author(s): Dirk Grosenick; Oliver Steinkellner; Heidrun Wabnitz; Rainer Macdonald; Thoralf Niendorf; Kathleen Cantow; Bert Flemming; Erdmann Seeliger
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Paper Abstract

We have developed a method to quantify hemoglobin concentration and oxygen saturation within the renal cortex by near-infrared spectroscopy. A fiber optic probe was used to transmit the radiation of three semiconductor lasers at 690 nm, 800 nm and 830 nm to the tissue, and to collect diffusely remitted light at source-detector separations from 1 mm to 4 mm. To derive tissue hemoglobin concentration and oxygen saturation of hemoglobin the spatial dependence of the measured cw intensities was fitted by a Monte Carlo model. In this model the tissue was assumed to be homogeneous. The scaling factors between measured intensities and simulated photon flux were obtained by applying the same setup to a homogeneous semi-infinite phantom with known optical properties and by performing Monte Carlo simulations for this phantom. To accelerate the fit of the tissue optical properties a look-up table of the simulated reflected intensities was generated for the needed range of absorption and scattering coefficients. The intensities at the three wavelengths were fitted simultaneously using hemoglobin concentration, oxygen saturation, the reduced scattering coefficient at 800 nm and the scatter power coefficient as fit parameters. The method was employed to study the temporal changes of renal hemoglobin concentration and blood oxygenation on an anesthetized rat during a short period of renal ischemia induced by aortic occlusion and during subsequent reperfusion.

Paper Details

Date Published: 25 March 2013
PDF: 8 pages
Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85781P (25 March 2013); doi: 10.1117/12.2000409
Show Author Affiliations
Dirk Grosenick, Physikalisch-Technische Bundesanstalt (Germany)
Oliver Steinkellner, Physikalisch-Technische Bundesanstalt (Germany)
Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany)
Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany)
Thoralf Niendorf, Max-Delbrück-Ctr. für Molekulare Medizin Berlin-Buch (Germany)
Kathleen Cantow, Charité Universitätsmedizin Berlin (Germany)
Bert Flemming, Charité Universitätsmedizin Berlin (Germany)
Erdmann Seeliger, Charité Universitätsmedizin Berlin (Germany)

Published in SPIE Proceedings Vol. 8578:
Optical Tomography and Spectroscopy of Tissue X
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca, Editor(s)

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