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Proceedings Paper

High contrast in vivo bioimaging using multiphoton upconversion in novel rare-earth-doped fluoride upconversion nanoparticles
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Paper Abstract

Upconversion in rare-earth ions is a sequential multiphoton process that efficiently converts two or more low-energy photons, which are generally near infrared (NIR) light, to produce anti-Stokes emission of a higher energy photon (e.g., NIR, visible, ultraviolet) using continuous-wave (cw) diode laser excitation. Here, we show the engineering of novel, efficient, and biocompatible NIRin-to-NIRout upconversion nanoparticles for biomedical imaging with both excitation and emission being within the “optical transparency window” of tissues. The small animal whole-body imaging with exceptional contrast (signal-to-noise ratio of 310) was shown using BALB/c mice intravenously injected with aqueously dispersed nanoparticles. An imaging depth as deep as 3.2-cm was successfully demonstrated using thick animal tissue (pork) under cw laser excitation at 980 nm.

Paper Details

Date Published: 22 February 2013
PDF: 4 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858814 (22 February 2013); doi: 10.1117/12.2000112
Show Author Affiliations
Guanying Chen, Univ. at Buffalo, SUNY (United States)
Harbin Institute of Technology (China)
Chunhui Yang, Harbin Institute of Technology (China)
Paras N. Prasad, Univ. at Buffalo, SUNY (United States)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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