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Proceedings Paper

Pheophorbides as photosensitizers for the photodynamic therapy of tumors
Author(s): Charles Tanielian; Christian Wolff; Masami Kobayashi
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Paper Abstract

Quantum yields for formation of singlet molecular oxygen have been measured for sodium pheophorbides (Na-Phdes) a and b in aqueous and non-aqueous media. Measurements have been made for both steady-state and pulsed laser excitation with the resultant singlet molecular oxygen being detected by photo-oxygenation reactions or time-resolved luminescence spectroscopy, respectively. Singlet oxygen production sensitized by Na-Phdes a or b is insignificant in aqueous media but occurs with a good efficiency in organic solvents. Plasmid DNA is efficiently photocleaved by Na-Phdes a and b in the absence of oxygen as well as in the presence of oxygen. Fluorescence microscopic observation shows a rapid incorporation of Na-Phde a into nuclei, mitochondria, and lysosome of human oral mucosa cells. In contrast Na-Phde b is incorporated only into the plasma membrane. The photodynamic activity of these pigments in living tissues is probably determined by the monomeric pigment molecules formed in hydrophobic cellular structures.

Paper Details

Date Published: 12 January 1995
PDF: 9 pages
Proc. SPIE 2325, Photodynamic Therapy of Cancer II, (12 January 1995); doi: 10.1117/12.199132
Show Author Affiliations
Charles Tanielian, Ecole Europeenne des Hautes Etudes des Industries (France)
Christian Wolff, Ecole Europeenne des Hautes Etudes des Industries (France)
Masami Kobayashi, Univ. of Tokyo (Japan)

Published in SPIE Proceedings Vol. 2325:
Photodynamic Therapy of Cancer II
Daniel Brault; Giulio Jori; Johan Moan; Benjamin Ehrenberg, Editor(s)

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