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Proceedings Paper

Properties of DNA-polyintercalating drugs studied by fluorescence lifetime techniques
Author(s): Stefan Winter; Liviu M. Popa
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Paper Abstract

Dimers of the fluorescent dye Oxazole Yellow (YOYO and POPO) are used for high sensitive DNA detection in gel electrophoresis. Upon binding to DNA they show a 3000 to 5000 fold enhancement of fluorescence. The binding constant of those dimers to dsDNA is between 108 M-1 and 109 M-1. This is due to the dye's ability to bisintercalate between adjacent DNA basepairs. We investigated the occurring forms of intercalation of YOYO to dsDNA in solutions of different ionic strength by fluorescence lifetime methods.

Paper Details

Date Published: 4 January 1995
PDF: 7 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198728
Show Author Affiliations
Stefan Winter, Institut fuer Molekulare Biotechnologie eV (Germany)
Liviu M. Popa, Medical and Pharmaceutical Institute of Cluj-Napoca (Romania)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg M.D.; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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