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Proceedings Paper

Rational use of fluorescent probes for the evaluation of intracellular ionic concentrations
Author(s): Pierre M. Viallet; Jean Vigo; Bruno Morelle; Jean-Marie Salmon
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Paper Abstract

The importance of the intracellular concentration and transmembrane flux of cations has led scientists to develop various techniques for the evaluation of intracellular concentrations of H+, Ca2+, Mg2+. Quantitative microfluorometry appears to be a powerful and noninvasive technique for it allows kinetic studies or mapping of ionic concentration in different cellular compartments as long as fluorescent markers specific of given cations are disposed of. Nevertheless, the previously published results sometimes look controversial. To minimize problems encountered by some scientists the following protocol is proposed: (1) careful and exhaustive determination of the physicochemical properties of the probe(s), (2) use of microspectrofluorometric techniques to resolve the complex intracellular fluorescence spectra into its components and calculate the molar fraction of each compound participating in the cellular fluorescence, (3) use of the same kind of algorithm to perform numerical image analysis.

Paper Details

Date Published: 4 January 1995
PDF: 14 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198724
Show Author Affiliations
Pierre M. Viallet, Univ. of Perpignan (France)
Jean Vigo, Univ. of Perpignan (France)
Bruno Morelle, Univ. of Perpignan (France)
Jean-Marie Salmon, Univ. of Perpignan (France)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg M.D.; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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