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Proceedings Paper

Laser-cytofluorescence microscopic image restoration by iterative deconvolution
Author(s): Chengqi Xu; Eric Maire; Serge Jacquey
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Paper Abstract

The aim of our study is to improve the performances of an optical microscope by image deconvolution technique to attain that of a confocal one in the field of laser cytofluorescence. The fluorescence of antigen lymphocyte sites marked by rhodamine is induced by a laser ((lambda) equals 543 nm) or a mercury vapor lamp. A set of scanned fluorescence images is acquired at different focal planes by a SIT camera, fitted on an optical ZEISS microscope (N.A. 1.25, X100). The entire system is controlled by a PC equipped with a MATROX-MVP-AT image processing card. Since an optical microscope has a more important focal depth than a confocal one, an improved iterative deconvolution algorithm of Van Cittert's has been used to artificially reduce the focal depth. In order to ensure the convergence of such an iterative algorithm, a new criterion for relaxation coefficient is defined through a theoretical study. The experiments show an improvement of 50% in the spatial x-y resolution of 30% in optical z axis gain. As regards our application, this processing brings our system to a comparable performance level as a confocal microscope.

Paper Details

Date Published: 4 January 1995
PDF: 8 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198723
Show Author Affiliations
Chengqi Xu, Univ. de Haute Alsace (France)
Eric Maire, Univ. de Haute Alsace (France)
Serge Jacquey, Univ. de Haute Alsace (France)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg M.D.; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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