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Proceedings Paper

Light-induced fluorescence of human colon tissue: dependence on histological and histochemical properties studied by means of a simplified model for simulation
Author(s): Renato Marchesini; Sabrina Fumagalli; Emanuele Pignoli; Adele Emilia Sichirollo; Stefano Tomatis; Silvana Di Palma; Marco Dal Fante; Pasquale Spinelli; Anna Cleta Croce; Giovanni F. Bottiroli
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Paper Abstract

In an attempt to evaluate the role of the histological and histochemical features of adenocarcinoma and normal tissue on their fluorescence spectral patterns, we developed a Monte Carlo model to simulate the fluorescence emission from tissue. According to histology, normal colon and adenocarcinoma were considered composed of three and two tissue layers, respectively (i.e., superficial epithelium, mucosa and submucosa; tumor mass and submucosa). Characterization of the fluorescence pattern of the tissue layers as well as of several histological components was performed by spectrofluorometric analysis on ex vivo samples. Results obtained from differently modelling the morphology and histological arrangement of colon suggest the following remarks. The decrease in fluorescence intensity observed in passing from normal to abnormal tissue could be ascribed mainly to a modification in morphology. When carcinomatous tissue is involved a modification in the spectral shape is also expected.

Paper Details

Date Published: 4 January 1995
PDF: 8 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198704
Show Author Affiliations
Renato Marchesini, Istituto Nazionale Tumori (Italy)
Sabrina Fumagalli, Istituto Nazionale Tumori (Italy)
Emanuele Pignoli, Istituto Nazionale Tumori (Italy)
Adele Emilia Sichirollo, Istituto Nazionale Tumori (Italy)
Stefano Tomatis, Istituto Nazionale Tumori (Italy)
Silvana Di Palma, Istituto Nazionale Tumori (Italy)
Marco Dal Fante, Istituto Nazionale Tumori (Italy)
Pasquale Spinelli, Istituto Nazionale Tumori (Italy)
Anna Cleta Croce, Univ. di Pavia (Italy)
Giovanni F. Bottiroli, Univ. di Pavia (Italy)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg M.D.; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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