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Proceedings Paper

Very compact all solid state fluorescence lifetime measurement system: preliminary results
Author(s): Rainer Erdmann; Gerald Kell; Rolf Krahl; Uwe Ortmann; Wolfgang Becker; Joerg Enderlein; Edgar O. Klose
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Paper Abstract

We will demonstrate the operation of the very compact all solid state fluorescence lifetime measurement system FLUO-TIME BQ 2759A. For this purpose we developed a new type of compact driving generator LD 4000 for a set of ps-laserdiodes with wavelengths between 630 nm and 690 nm, which will produce sub 50 ps pulses with up to 200 mW peak power and 3 MHz repetition rate. Using this miniaturized excitation source we are able to investigate a lot of red and NIR dyes. The fluorescence signal will be detected with single photon counting sensitivity by an ultrafast photomultiplier tube with only the size of the transistor (TO8 housing). Spectral resolution is given by a set of bandpass filters or a compact monochromator. With our recently introduced time correlated single photon counting (TCSPC) electronics SPC 300 (a PC-plug-in-card) we have a powerful instrument for data acquisition with highest data throughput. The instrumental response time (IRF) of the complete measurement system is less than 250 ps, allowing the investigation of fluorescence decay time components down to 25 ps using out deconvolution and analysis software package PHYSFIT. This performance can be improved to less than 90 ps IRF using a microchannel plate photomultiplier tube (MCP-PMT) detector. In this paper we demonstrate also the first practical application of this system to standard fluorescence dyes (oxazine, rhodamin).

Paper Details

Date Published: 22 December 1994
PDF: 9 pages
Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); doi: 10.1117/12.197519
Show Author Affiliations
Rainer Erdmann, BioQuant Ltd. (Germany)
Gerald Kell, BioQuant Ltd. (Germany)
Rolf Krahl, BioQuant Ltd. (Germany)
Uwe Ortmann, BioQuant Ltd. (Germany)
Wolfgang Becker, Becker and Hickl Ltd. (Germany)
Joerg Enderlein, BioQuant Ltd. (Germany)
Edgar O. Klose, BioQuant Ltd. (Germany)

Published in SPIE Proceedings Vol. 2328:
Biomedical Optoelectronic Devices and Systems II
Nathan I. Croitoru; Norbert Kroo; Mitsunobu Miyagi; Riccardo Pratesi; Juergen M. Wolfrum, Editor(s)

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