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Proceedings Paper

Contractile properties of myofibrillar bundles and fusion of sarcolemma vesicles of normal and diseased skeletal muscle using UV-laser microbeam dissection and IR-optical trapping
Author(s): Claudia Veigel; Dorothea von Pfeil; Abdellah Harim; Rainer H.A. Fink
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Paper Abstract

Applying ultraviolet-laser microdissection enabled us to obtain very small myofibrillar preparations (e.g. 3 micrometers diameter) of normal and diseased muscle fibers which develop forces in the range of nano to micronewton too small to be measured with conventional force transducers. For the present study we built a very sensitive force transducer based on determining the force induced light beam deflection of a 50 micrometers optical fiber, connected to a 635 nm diode laser and detected by two photodiodes at two oppositely positioned 100 micrometers optical fibers to measure forces of smallest myofibrillar preparations. Also, for electrophysiological and stability studies selected vesicles of normal and myopathic fibers were trapped by the infrared-microbeam, brought into close membrane contact with other vesicles and fused by single pulses of the ultraviolet-microbeam. This approach allows for the first time to study membrane properties of normal and myopathic tissue in one preparation under the same intra and extravesicular medium conditions. These 'hybrid' vesicles should be of particular importance for studies of structural stability and electrophysiological properties or lateral mobility of ion channels, e.g. in presence or absence of the membrane-bound cytoskeleton dystrophin-glycoprotein complex which is less or not at all expressed in mdx-mice and Duchenne/Becker patients.

Paper Details

Date Published: 22 December 1994
PDF: 12 pages
Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); doi: 10.1117/12.197516
Show Author Affiliations
Claudia Veigel, Ruprecht-Karls-Univ. (Germany)
Dorothea von Pfeil, Ruprecht-Karls-Univ. (Germany)
Abdellah Harim, Ruprecht-Karls-Univ. (Germany)
Rainer H.A. Fink, Ruprecht-Karls-Univ. (Germany)

Published in SPIE Proceedings Vol. 2328:
Biomedical Optoelectronic Devices and Systems II
Nathan I. Croitoru; Norbert Kroo; Mitsunobu Miyagi; Riccardo Pratesi; Juergen M. Wolfrum, Editor(s)

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