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Proceedings Paper

Identification of haematic cells by spectroscopic analysis of the intrinsic fluorescence
Author(s): Monica Monici; Giovanni Agati; Franco Fusi; Pietro Antonio Bernabei; Roberto Caporale; Pierluigi Rossi Ferrini; Anna Cleta Croce; Giovanni F. Bottiroli; Stefano Cioncolini; Alberto Innocenti; Riccardo Pratesi
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Paper Abstract

The determination of blood cell composition has been a valuable tool in diagnoses. In particular, both total and differential counts are considered the basic parameters that characterize the leukocyte population. Since 100 years ago, manual techniques were introduced that allow a morphological examination of blood smears. At present, the automated analysis has been proved to be particularly difficult to standardize. In fact, the identification and count of the five leukocyte populations are not completely solved problems in routine methods for hematological analysis. Optoelectronics could have a decisive role in the development of new techniques that can ensure characteristics of automation, reliability, accuracy and rapidity of execution. Fluorescence spectroscopy techniques could represent a valid approach. Recently, the evaluation of tissue and cell autofluorescence has been applied to the diagnosis of solid tissue neoplasies. In this work, we have considered the possibility to develop a reliable method of leukocyte analysis based on their intrinsic fluorescence emission properties. The study has been performed by applying both spectrofluorometric techniques to enriched suspensions of cells and microspectrofluorometric techniques to single leukocytes. The results obtained have shown the possibility to recognize some cell populations on the grounds of the intrinsic fluorescence characteristics.

Paper Details

Date Published: 22 December 1994
PDF: 8 pages
Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); doi: 10.1117/12.197511
Show Author Affiliations
Monica Monici, Istituto di Elettronica Quantistica/CNR and Univ. di Firenze (Italy)
Giovanni Agati, Istituto di Elettronica Quantistica/CNR (Italy)
Franco Fusi, Istituto di Elettronica Quantistica/CNR (Italy)
Pietro Antonio Bernabei, Univ. di Firenze (Italy)
Roberto Caporale, Univ. di Firenze (Italy)
Pierluigi Rossi Ferrini, Univ. di Firenze (Italy)
Anna Cleta Croce, Ctr. di Studio per l`Istochimica/CNR (Italy)
Giovanni F. Bottiroli, Ctr. di Studio per l`Istochimica/CNR (Italy)
Stefano Cioncolini, SEAC srl (Italy)
Alberto Innocenti, SEAC srl (Italy)
Riccardo Pratesi, Univ. di Firenze (Italy)

Published in SPIE Proceedings Vol. 2328:
Biomedical Optoelectronic Devices and Systems II
Nathan I. Croitoru; Norbert Kroo; Mitsunobu Miyagi; Riccardo Pratesi; Juergen M. Wolfrum, Editor(s)

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