Share Email Print

Proceedings Paper

Detector for two-dimensional time-resolved photon counting
Author(s): Malte Koellner; Peter Fischer; John S. McCaskill
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

We are developing a novel counting detector with high spatial and ultrahigh temporal resolution for 2D single molecule detection in solution. It is based on an image intensifier optically coupled to a position sensitive detector for photon-bursts. An electric trigger signal taken from the image intensifier provides sub-nanosecond temporal resolution. The detector has the following features: true single photon counting with a quantum efficiency of approximately 20% (photo cathode), spatial resolution approximately 200 X 200 pixels, temporal resolution approximately 100 ps, and a maximum count rate of several million counts per second. The design of the detector is inspired by the technique of time correlated single photon counting (TCSPC). It allows the simultaneous determination of the position of fluorescing molecules and their fluorescence lifetime.

Paper Details

Date Published: 22 December 1994
PDF: 7 pages
Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); doi: 10.1117/12.197503
Show Author Affiliations
Malte Koellner, Institute for Molecular Biotechnology (Germany)
Peter Fischer, Institute for Molecular Biotechnology (Germany)
John S. McCaskill, Institute for Molecular Biotechnology (Germany)

Published in SPIE Proceedings Vol. 2328:
Biomedical Optoelectronic Devices and Systems II
Nathan I. Croitoru; Norbert Kroo; Mitsunobu Miyagi; Riccardo Pratesi; Juergen M. Wolfrum, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?