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Proceedings Paper

Particle size and scattering cross-section effects in laser Doppler electrophoresis of biological cells
Author(s): Vadim L. Kononenko
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Paper Abstract

The influence of the correlation between the size and electrophoretic mobility of particles on the shape of laser Doppler electrophoresis spectrum is considered. The theory of spectrum shape in the presence of such correlation is developed. It is shown that the oscillatory dependence of a light scattering cross-section of large particles on their size may distort substantially the spectrum shape corresponding solely to the electrophoretic mobility distribution in a particles system. Even the appearance of `false' line structure in the spectrum is possible for polydisperse particles systems with smooth distribution curves but with a high degree of size-electrophoretic mobility correlation. The theory explains naturally the oscillation structure observed in Doppler electrophoresis spectra of certain large cells.

Paper Details

Date Published: 2 January 1995
PDF: 10 pages
Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); doi: 10.1117/12.197463
Show Author Affiliations
Vadim L. Kononenko, N.N. Semenov Institute of Chemical Physics (Russia)

Published in SPIE Proceedings Vol. 2370:
5th International Conference on Laser Applications in Life Sciences
Pavel A. Apanasevich; Nikolai I. Koroteev; Sergei G. Kruglik; Victor N. Zadkov, Editor(s)

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