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Proceedings Paper

Scanning transmission and computer-aided volumic electron microscopy: 3-D modeling of entire cells by electronic imaging
Author(s): Christophe Bron; Philip Gremillet; D. Launay; Michel Jourlin; H. P. Gautschi; Thomas Baechi; Joerg Schuepbach
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Paper Abstract

The digital processing of electron microscopic images from serial sections containing laser-induced topographical references allows a 3-D reconstruction at a depth resolution of 30 to 40 nm of entire cells by the use of image analysis methods, as already demonstrated for Transmission Electron Microscopy (TEM) coupled with a video camera. We decided to use a Scanning Transmission Electron Microscope (STEM) to get higher contrast and better resolution at medium magnification. The scanning of our specimens at video frequencies is an attractive and easy way to link a STEM with an image processing system but the hysteresis of the electronic spools responsible for the magnetic deviation of the scanning electron beam induces deformations of images which have to be modelized and corrected before registration. Computer algorithms developed for image analysis and treatment correct the artifacts caused by the use of STEM and by serial sectioning to automatically reconstruct the third dimension of the cells. They permit the normalization of the images through logarithmic processing of the original grey level infonnation. The automatic extraction of cell limits allows to link the image analysis and treatments with image synthesis methods by minimal human intervention. The surface representation and the registered images provide an ultrastructural data base from which quantitative 3-D morphological parameters, as well as otherwise impossible visualizations, can be computed. This 3-D image processing named C.A.V.U.M. for Computer Aided Volumic Ultra-Microscopy offers a new tool for the documentation and analysis of cell ultrastructure and for 3-D morphometric studies at EM magnifications. Further, a virtual observer can be computed in such a way as to simulate a visit of the reconstructed object.

Paper Details

Date Published: 1 May 1990
PDF: 7 pages
Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); doi: 10.1117/12.19541
Show Author Affiliations
Christophe Bron, Univ. of Zurich (Switzerland)
Philip Gremillet, Univ. de Saint-Etienne (France)
D. Launay, Computimage GmbH (Switzerland)
Michel Jourlin, Univ. de Saint-Etienne (France)
H. P. Gautschi, Univ. of Zurich (Switzerland)
Thomas Baechi, Univ. of Zurich (Switzerland)
Joerg Schuepbach, Univ. of Zurich (Switzerland)

Published in SPIE Proceedings Vol. 1245:
Biomedical Image Processing
Alan Conrad Bovik; William E. Higgins, Editor(s)

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