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Proceedings Paper

Modeling of 3D imaging in Brightfield microscope systems
Author(s): Colin J. R. Sheppard; Min Gu
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Paper Abstract

The way in which a reflection or transmission microscope image depends on the variations in complex refractive index in the object is considered. Reflection objects including rough surfaces and stratified media are discussed, and a generalized form for the scattering potential for reflection imaging in the Born approximation proposed. Reconstruction of the refractive index variation in a stratified medium is considered. Transmission imaging formation based on the Born and Rytov approximations is discussed. These properties are important for interpretation of the resulting images, and also as a basis for digital restoration methods.

Paper Details

Date Published: 30 September 1994
PDF: 7 pages
Proc. SPIE 2302, Image Reconstruction and Restoration, (30 September 1994); doi: 10.1117/12.188053
Show Author Affiliations
Colin J. R. Sheppard, Univ. of Sydney (Australia)
Min Gu, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 2302:
Image Reconstruction and Restoration
Timothy J. Schulz; Donald L. Snyder, Editor(s)

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