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Proceedings Paper

Fluorescence in normal and carcinoma bronchial tissue
Author(s): Jianan Y. Qu; Calum E. MacAulay; Stephen Lam; Haishan Zeng; Branko Palcic
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Paper Abstract

Optical spectroscopy techniques are frequently used for non-invasive detection of abnormal tissues. For these techniques it is necessary to know the tissue optical properties, such as absorption and scattering coefficients, anisotropy factor and intrinsic fluorescence yield. Using a collimated white light illumination system, polarized on-axis light detection and integrating sphere techniques, optical properties of normal and cancerous bronchial tissue were measured for the wavelength range of 400 to 700 nm. Fluorescence microscopy was used to measure the fluorescence excitation function and spectra of bronchial tissue as a function of depth into the tissue. The distribution of 442 nm He-Cd laser light inside the tissue was calculated by Monte Carlo simulation. The propagation of excited green, yellow and red fluorescence inside the bronchial tissue was simulated using the Monte Carlo model and fluorescence escape functions were calculated for various depths. By comparing the total fluorescence of normal and carcinoma tissue calculated from the Monte Carlo simulation with in vivo fluorescence measurement results, the fluorescence characteristics of normal and abnormal bronchial tissue could be explained.

Paper Details

Date Published: 17 August 1994
PDF: 11 pages
Proc. SPIE 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards, (17 August 1994); doi: 10.1117/12.182938
Show Author Affiliations
Jianan Y. Qu, Xillix Technologies Corp. and British Columbia Cancer Research Ctr. (Hong Kong)
Calum E. MacAulay, British Columbia Cancer Research Ctr. (Canada)
Stephen Lam, British Columbia Cancer Research Ctr. (Canada)
Haishan Zeng, British Columbia Cancer Research Ctr. (Canada)
Branko Palcic, British Columbia Cancer Research Ctr. (Canada)

Published in SPIE Proceedings Vol. 2134:
Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards
Steven L. Jacques; David H. Sliney; Michael Belkin M.D., Editor(s)

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