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Proceedings Paper

Conformation, motion, and base-to-base excitation energy transfer in DNA
Author(s): Thomas M. Nordlund; Daguang Xu; Kervin O. Evans
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Paper Abstract

Absorption and fluorescence spectra of the DNA duplex decamer d[CTGA(2AP)TTCAG]2 have been measured as a function of temperature. Emission is dominated by the 2-aminopurine (2AP) base. A fluorescence excitation band near 260 to 270 nm disappears near the duplex melting temperature but reappears at higher temperatures, indicating singlet-singlet energy transfer occurs between normal DNA bases and the 2AP base in the B-helical conformation and to a lesser extent in the structurally-mobile melted conformation. The efficiency of transfer is 4 to 5% at 4 degree(s)C under the assumption of a single acceptor; preferential transfer to a low-fluorescence conformation of 2AP would imply no more than a two-times greater transfer efficiency. Emission spectra excited at 260 nm (energy transfer band), 330 nm (basepaired conformation of 2AP) and 305 nm (nonpaired 2AP conformation) appear to indicate transfer occurs from normal DNA bases to both 2AP conformers.

Paper Details

Date Published: 17 August 1994
PDF: 10 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182773
Show Author Affiliations
Thomas M. Nordlund, Univ. of Alabama/Birmingham (United States)
Daguang Xu, Univ. of Alabama/Birmingham (United States)
Kervin O. Evans, Univ. of Alabama/Birmingham (United States)

Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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