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Proceedings Paper

Internal fluorescent probes for studying irregular and metastable DNA structures
Author(s): Theodore E. Carver; David P. Millar
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Paper Abstract

Time-resolved fluorescence anisotropy decay measurements are useful for characterizing differences in the local structural environment of probes attached to DNA molecules. We synthesized oligonucleotides with dansyl probes covalently attached to uridine near the center of a 17 base oligonucleotide. The fluorescence properties of these DNAs hybridized to complementary DNA were examined using time-correlated single photon counting. We present preliminary data indicating changes in the probe anisotropy in the presence of trifluoroethanol, divalent zinc cations, and under conditions promoting the formation of a hairpin junction at the site of probe incorporation. The results of these perturbations indicate that this approach will be useful for investigating site-specific and global structural phenomena in DNA.

Paper Details

Date Published: 17 August 1994
PDF: 6 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182757
Show Author Affiliations
Theodore E. Carver, Scripps Research Institute (United States)
David P. Millar, Scripps Research Institute (United States)

Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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