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Proceedings Paper

Interaction of 7-aminoactinomycin D with single- and double-stranded DNA oligonucleotides
Author(s): Susan A. Bailey; David E. Graves; Maurice R. Eftink
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Paper Abstract

7-Aminoactinomycin D is a potent inhibitor of RNA polymerase and it has been demonstrated to bind to a single stranded DNA. Wadkins and Jovin showed that there is a 20 to 40 fold enhancement in the fluorescence of 7-aminoactinomycin D upon binding to certain single stranded DNA sequences. We report studies of the thermodynamics of the binding of the parent drug, actinomycin D, to single and double stranded DNA. A five-fold range of association constants is found for the different binding sequences that we have investigated. We will correlate these thermodynamic studies with measurements of the fluorescence enhancement, lifetime, and rotational correlation time of the drug bound to the various single and double stranded oligonucleotides.

Paper Details

Date Published: 17 August 1994
PDF: 7 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182756
Show Author Affiliations
Susan A. Bailey, Univ. of Mississippi (United States)
David E. Graves, Univ. of Mississippi (United States)
Maurice R. Eftink, Univ. of Mississippi (United States)

Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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