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Proceedings Paper

Aggregation and deformation of red blood cells as probed by a laser light scattering technique in a concentrated suspension: methodology
Author(s): Amir H. Gandjbakhche; Pierre Mills; Patrick Snabre; Jacques Dufaux
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Paper Abstract

A light scattering technique is presented which investigates the backscattered and transmitted flux of He-Ne laser light illuminating a concentrated suspension of red blood cells (RBC) submitted to a simple shear flow. Our experiments show that the angular distributions of scattered light are closely related to the state of the suspension (at rest, or submitted to a simple shear flow) and the rheological parameters of the suspension such as viscosity and volume concentration. Transmitted and reflected light measurements demonstrated reproducible and predictable changes in scattering cross sections of oriented and deformed RBCs by shear flow. Additionally, it is shown that the scattering cross-section of aggregated RBCs is different from disaggregated RBCs. A theoretical model, substantiated by Monte- Carlo simulations, is proposed which relates the changes in shape of the angular distribution of scattered light to the rate of aggregation and the gradients of deformation of RBCs.

Paper Details

Date Published: 21 July 1994
PDF: 10 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180775
Show Author Affiliations
Amir H. Gandjbakhche, National Institutes of Health (Israel)
Pierre Mills, Univ. Paris VII (France)
Patrick Snabre, Lab. du Genie des Procedes et des Materiaux/CNRS (France)
Jacques Dufaux, Univ. Paris VII (France)

Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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