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Proceedings Paper

Substrate-induced quenching of a fluorophore and its application in biosensor development
Author(s): Ashutosh Sharma; Nigel S. M. Quantrill
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Paper Abstract

A novel process called substrate induced quenching (SIQ) and a mathematical derivation of an empirical relation describing it are presented. Based on this a model is proposed for a dehydrogenase enzymatic system that includes the effects due to dynamic/static quenching of the fluorophore by either the enzyme substrate or the product. The validity of the model is shown by comparison with experimental results for the SIQ based ethanol assay involving the alcohol dehydrogenase enzyme. Various applications of this novel technique are discussed.

Paper Details

Date Published: 28 July 1994
PDF: 15 pages
Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); doi: 10.1117/12.180764
Show Author Affiliations
Ashutosh Sharma, Cranfield Univ. (United Kingdom)
Nigel S. M. Quantrill, Cranfield Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 2131:
Biomedical Fiber Optic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir; Fred P. Milanovich, Editor(s)

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