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Proceedings Paper

Single-mode tapered optical fiber immunosensor I: characterization with model analytes
Author(s): Zoe M. Hale; Robert S. Marks; C. R. Lowe; Frank P. Payne
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Paper Abstract

A novel single mode tapered optical fiber loop biochemical sensor based on fluorescence spectroscopy has been developed. The fundamental fiber mode propagating through the tapered portion of the waveguide has evanescent fields which penetrate into the aqueous environment where the biochemical recognition event occurs. The model measurands were conjugated with a fluorescent dye, fluorescein isothiocyanate. When excited by the input laser light from the near end of the taper, generated fluorescence is coupled into the guided mode of the fiber and collected at the far end of the taper. Several radioactive and fluorescent quantification methods have been explored to determine the density of available binding sites immobilized on the fiber, and thus the ultimate sensitivity of the device. A generic avidin- biotin system has been tested as a model immunological diagnostic system. The high sensitivity of a single mode tapered loop device combined with a simple immobilization method provides a powerful tool for performing immunoassays.

Paper Details

Date Published: 28 July 1994
PDF: 11 pages
Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); doi: 10.1117/12.180745
Show Author Affiliations
Zoe M. Hale, Univ. of Cambridge (United Kingdom)
Robert S. Marks, Univ. of Cambridge (United Kingdom)
C. R. Lowe, Univ. of Cambridge (United Kingdom)
Frank P. Payne, Univ. of Cambridge (United Kingdom)

Published in SPIE Proceedings Vol. 2131:
Biomedical Fiber Optic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir; Fred P. Milanovich, Editor(s)

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