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Proceedings Paper

In-situ detection of viral nucleic acids using fluorescent probes
Author(s): Richard M. Donovan
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Paper Abstract

The objective of this work was to develop and improve technologies in cytometry and molecular biology for the specific in situ detection of viral nucleic acids. The major application for this system was the detection and measurement of individual cells stained specifically for the Human Immunodeficiency Virus (HIV) in patients with Acquired Immune Deficiency Syndrome (AIDS). Staining procedures used nucleic acid either directly or indirect labeled with enzymes or fluorescent probes. A cytometry system was used to acquire digitized images of labeled cells and determine their individual staining density or intensity. Efforts are underway to improve the sensitivity of these assays using time-resolved methods.

Paper Details

Date Published: 1 July 1990
PDF: 5 pages
Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); doi: 10.1117/12.17802
Show Author Affiliations
Richard M. Donovan, Univ. of California/Davis (United States)

Published in SPIE Proceedings Vol. 1206:
New Technologies in Cytometry and Molecular Biology
Gary C. Salzman, Editor(s)

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