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Proceedings Paper

Multicolor fluorescence imaging system for tissue diagnostics
Author(s): Stefan Andersson-Engels; Jonas Johansson; Sune Svanberg
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Paper Abstract

A multicolor fluorescence imaging system for tissue diagnostics has been constructed. Examples given to illustrate the system performance are atherosclerotic plaque lesions from human artery samples and a malignant rat brain tumor model. The system simultaneously monitors fluorescence images at four different wavelengths, enabling spatial as well as spectral information to be extracted. By selection of band pass filters an artificial image of the lesion under study can be formed, based on an optimal contrast function of four fluorescence intensities. The image is constructed in a computer and displayed in false colors on a monitor. Pulsed excitation light from an N2 laser provides the possibility of using a gated detector, and in this way suppress room light to an undetectable level. Images can thus be recorded under white light illumination during visual inspection, increasing the usefulness of the system.

Paper Details

Date Published: 1 August 1990
PDF: 11 pages
Proc. SPIE 1205, Bioimaging and Two-Dimensional Spectroscopy, (1 August 1990); doi: 10.1117/12.17794
Show Author Affiliations
Stefan Andersson-Engels, Lund Institute of Technology (Sweden)
Jonas Johansson, Lund Institute of Technology (Sweden)
Sune Svanberg, Lund Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 1205:
Bioimaging and Two-Dimensional Spectroscopy
Louis C. Smith, Editor(s)

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